Molecular characterization of cDNA for phospholipase A2-activating protein
Identifieur interne : 002607 ( Main/Exploration ); précédent : 002606; suivant : 002608Molecular characterization of cDNA for phospholipase A2-activating protein
Auteurs : A. K. Chopra [États-Unis] ; D. A. Ribardo [États-Unis] ; T. G. Wood [États-Unis] ; D. J. Prusak [États-Unis] ; X.-J. Xu [États-Unis] ; J. W. Peterson [États-Unis]Source :
- BBA - Gene Structure and Expression [ 0167-4781 ] ; 1999.
English descriptors
- Teeft :
Abstract
Abstract: A phospholipase A2-activating protein (PLAP) cDNA was cloned and sequenced from a human monocyte cDNA library, and expressed as a histidine-tagged fusion protein. The DNA-deduced aa sequence of human PLAP was 80,826 Da; however, SDS-PAGE analysis revealed a 72-74 kDa protein which matched the size of native PLAP from human monocytes. Anti-sense plap oligonucleotide blocked cholera toxin-induced release of 3H-labeled arachidonic acid from cells, indicating a potential role for PLAP in regulating phospholipase A2 activity.
Url:
DOI: 10.1016/S0167-4781(98)00249-8
Affiliations:
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Xu</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, TX 77555-1070</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Peterson, J W" sort="Peterson, J W" uniqKey="Peterson J" first="J. W." last="Peterson">J. W. 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The DNA-deduced aa sequence of human PLAP was 80,826 Da; however, SDS-PAGE analysis revealed a 72-74 kDa protein which matched the size of native PLAP from human monocytes. Anti-sense plap oligonucleotide blocked cholera toxin-induced release of 3H-labeled arachidonic acid from cells, indicating a potential role for PLAP in regulating phospholipase A2 activity.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Chopra, A K" sort="Chopra, A K" uniqKey="Chopra A" first="A. K." last="Chopra">A. K. Chopra</name></region><name sortKey="Chopra, A K" sort="Chopra, A K" uniqKey="Chopra A" first="A. K." last="Chopra">A. K. Chopra</name><name sortKey="Peterson, J W" sort="Peterson, J W" uniqKey="Peterson J" first="J. W." last="Peterson">J. W. Peterson</name><name sortKey="Prusak, D J" sort="Prusak, D J" uniqKey="Prusak D" first="D. J." last="Prusak">D. J. 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